Immunofluorescent analysis of CD69 (green) in PHA-M (2% v/v, Product # 10576-015) treated Raji cells. Cells were blocked with blocking buffer (Product # 37525) for 30 min at room temparture and then stained with CD69 hamster monoclonal antibody (Product # MA1-207) at a dilution of 1:100 for 1 hour on ice. After washing with ice-cold PBS for 3 times, the cells were then stained with DyLight™ 488 Goat anti-hamster (Armenian) IgG antibody for 1 hour on ice. The cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature. Nuclei (blue) were counterstained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.